iO Biotin is the rate limiting nutrient in maintaining healthy hoofs. iO Biotin is a water soluble B-group vitamin, which cannot be stored in the body and is lost through urination and sweating, therefore requiring daily supplementation. iO Biotin is essential for repairing hoof defects, as well as being essential for normal skin, hair and coat development. The recommended dose rate is 15g per 100kg of body weight twice daily for medium (400kg) and heavy horses (600kg). 90g once daily for light horses (300kg) and 60g twice daily for ponies and weanlings. Active Ingredients: Biotin 180mg/kg in a calcium base. Directions for Use: Mix Biotin powder into the feed. Treatment with iO Biotin should continue for 6-9 months for the maximum therapeutic effect.